Introduction. Hemodynamic adaptation to change of body position determines proper organ perfusion. Impedance cardiography (ICG) enables the non‑invasive assessment of cardiovascular response to orthostatic stress, measuring e.g.: mean blood pressure (MBP), heart rate (HR), stroke volume index (SI), systemic vascular resistance index (SVRI) and thoracic fluid content (TFC). For the correct interpretation of ICG, it is very important to determine the impact of body position on those parameters. Therefore, the aim of the study was to evaluate the impact of verticalization on the hemodynamic profile. Methods. 30 patients (aged 22–74 years) underwent a 20‑minute ICG in different body positions and hemodynamic parameters were recorded. Results. The change of body position from supine to sitting was associated with increased MBP, HR, SVRI and decreased SI and TFC. The most significant change of MBP, HR and TFC was observed in 1st minute after the position change, while SI and SVRI were changing gradually. Return to horizontal position indicated the return of the parameters to their baseline values. Conclusions. Body position has significant impact on the hemodynamic parameters measured by ICG. Sequential testing should be performed in the same body position.