The increasing popularity of travel to tropical regions has led to a rise in mixed intestinal infections among returning travelers. These infections, caused by a combination of bacterial, viral, and parasitic pathogens, present complex diagnostic and treatment challenges. Travel patterns are evolving, with a growing number of individuals venturing into tropical regions. This trend is likely to continue, highlighting the need for a greater understanding of mixed intestinal infections in this population. A diverse range of pathogens can cause mixed intestinal infections, with Enteroadherent E. coli, Enteropathogenic E. coli, the protozoan Giardia intestinalis, noroviruses, and the stramenopile Blastocystis hominis being particularly prevalent. The interplay between these organisms can lead to a broad spectrum of clinical manifestations. Co-infections with multiple pathogens are common in mixed intestinal infections and can significantly worsen the severity of symptoms. Understanding the mechanisms and interactions of co-infections is crucial for effective management. Diagnosing mixed intestinal infections requires a multifaceted approach, combining clinical presentation, stool analysis, and advanced tests such as polymerase chain reaction. Early and accurate diagnosis is essential for prompt and appropriate treatment.