Introduction. Regular physical activity increases the activity of parasympathetic nervous system, changing the sympathetic–parasympathetic balance towards the latter. Aim. The assessment of baroreflex sensitivity (BRS) at baseline and during provocative tests in groups of soldiers with average (PW) and above average (PPW) physical fitness in order to identify the BRS parameters that differentiate the two groups. Methods. 70 soldiers with PW and 124 healthy soldiers with PPW aged 18–45 years underwent the test. An assessment of BRS at baseline and during provocative tests: controlled breathing test (CBT), handgrip test (CBT) and tilt test (TT) were performed. Results. The most distinguished inter‑group differences concerned baseline BRS assessment. Alfa LF and alfa HF values were higher in the PPW group. During CBT and HGT, no statistically significant differences were noted between the groups. During TT, the differences in PPW group were subtle. Conclusions. The assessment of AUN with BRS enables to differentiate individuals with different level of physical fitness. The most distinguished differences in BRS parameters were registered at baseline. The assessment of BRS during provocative tests does not show significant inter‑group differences enough to consider its performance justified.