Introduction. FemtoLASIK is currently the world’s most common laser surgery to correct sight defects. Puropose. Evaluation of influence of thickness the corneal flap made as an effect of FemtoLASIK surgery on tear film parameters and ocular surface condition in patients with myopia. Materials and methods. Prosepective, interventive and open study involved 143 patients (143 eyes, 80 females, av. age 27.6+–5.9 yrs) with myopia (-0.75 to −7.75 diopters), mean SE of 4.76+–1.63 D. The patients underwent FemtoLASIK surgery with 110 or 140 microns flap created. Schirmer I and II, TBUT and fluorescein eye stain tests were conducted before the surgery and on week 1 and months 1, 2, 4 and 6 after the surgery. Results. All the evaluated tear film parameters altered after the surgery and remained lower throughout the whole 6‑month follow‑up period. Conclusions. Tear film alterations and dry eye syndrome are more frequent after thicker corneal flap creation and in females.